Professional China Primer Purification Machine - Purification Equipment for Oligo purification – Honya

Application:


Product Detail

Product Tags

Related Video

Feedback (2)

Our staff through skilled training. Skilled skilled knowledge, potent sense of company, to satisfy the provider requirements of consumers for Recombinant Dna Vaccine, Dna Strand Synthesis, Cap B, Our tenet is "Reasonable prices, efficient production time and best service" We hope to cooperate with more customers for mutual development and benefits.
Professional China Primer Purification Machine - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


Product detail pictures:

Professional China Primer Purification Machine - Purification Equipment for Oligo purification – Honya detail pictures


Related Product Guide:

Being supported by an state-of-the-art and skilled IT team, we could supply technical support on pre-sales & after-sales service for Professional China Primer Purification Machine - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Czech republic, Barbados, Brasilia, Our company has already had a lot of top factories and professional technology teams in China, offering the best products, techniques and services to worldwide customers. Honesty is our principle, professional operation is our work, service is our goal, and customers' satisfaction is our future!
  • The factory has advanced equipment, experienced staffs and good management level, so product quality had assurance, this cooperation is very relaxed and happy!
    5 Stars By Mag from Paris - 2018.07.12 12:19
    Company director has very rich management experience and strict attitude, sales staff are warm and cheerful, technical staff are professional and responsible,so we have no worry about product,a nice manufacturer.
    5 Stars By Norma from Guyana - 2018.05.13 17:00
    Write your message here and send it to us