Manufacturer for Primer Purification Methods – Purification Equipment for Oligo purification – Honya

Application:


Product Detail

Product Tags

Related Video

Feedback (2)

We goal to understand excellent disfigurement from the manufacturing and supply the top support to domestic and abroad clients wholeheartedly for Primer Purification Instrument, Desalting Oligonucleotides, Replicated Dna Sequence, We can do your customized order to meet your own satisfactory! Our company sets up several departments, including production department, sales department, quality control department and sevice center, etc.
Manufacturer for Primer Purification Methods – Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


Product detail pictures:

Manufacturer for Primer Purification Methods – Purification Equipment for Oligo purification – Honya detail pictures


Related Product Guide:

We are experienced manufacturer. Wining the majority of the crucial certifications of its market for Manufacturer for Primer Purification Methods – Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Swedish, Greek, Israel, With more and more Chinese products and solutions around the world, our international business is developing rapidly and economic indicators big increase year by year. We have enough confidence to supply you both better solutions and service, because we've been more and more powerful, specialist and experience in domestic and international.
  • The company's products can meet our diverse needs, and the price is cheap, the most important is that the quality is also very nice.
    5 Stars By Arlene from Guatemala - 2018.10.01 14:14
    The customer service reprersentative explained very detailed, service attitude is very good, reply is very timely and comprehensive, a happy communication! We hope to have a opportunity to cooperate.
    5 Stars By Dominic from Jersey - 2017.08.18 11:04
    Write your message here and send it to us