High Quality Primer Purification - Purification Equipment for Oligo purification – Honya
High Quality Primer Purification - Purification Equipment for Oligo purification – Honya Detail:
Specification
| Compatible boards | 1, 3, 5, 8. |
| Filtration | blow filtration, suction filtration |
| Number of injection ports | 5, 6, 7, 8, 9, 10. |
| Compatible plate types | C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate |
| Module | single axis or dual axis |
| Voltage | 220V |
| Warranty | 1 year |
| Custom | Accepted |
Different ways of Purification
1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.
2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.
This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.
3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.
This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.
Product detail pictures:
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Well-run gear, qualified revenue workforce, and superior after-sales companies; We've been also a unified huge loved ones, anyone persist with the organization benefit "unification, determination, tolerance" for High Quality Primer Purification - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Poland, Riyadh, Philadelphia, Our products are exported worldwide. Our customers are always satisfied with our reliable quality, customer-oriented services and competitive prices. Our mission is "to continue to earn your loyalty by dedicating our efforts to the constant improvement of our products and services in order to ensure the satisfaction of our end-users, customers, employees, suppliers and the worldwide communities in which we cooperate".
By Odelette from Myanmar - 2018.10.01 14:14
Company director has very rich management experience and strict attitude, sales staff are warm and cheerful, technical staff are professional and responsible,so we have no worry about product,a nice manufacturer.
By Lillian from Macedonia - 2018.12.10 19:03
