Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya

Application:


Product Detail

Product Tags

Related Video

Feedback (2)

"Sincerity, Innovation, Rigorousness, and Efficiency" would be the persistent conception of our enterprise with the long-term to build with each other with consumers for mutual reciprocity and mutual advantage for Column Dna, Oligo Purification, Primer Synthesis Use Sieve Plates, "Quality", "honesty" and "service" is our principle. Our loyalty and commitments remain respectfully at your service. Contact Us Today For further information, contact us now.
Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


Product detail pictures:

Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya detail pictures


Related Product Guide:

We have been ready to share our knowledge of advertising and marketing worldwide and recommend you suitable products and solutions at most competitive price ranges. So Profi Tools supply you best benefit of money and we're ready to create with each other with Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Netherlands, Saudi Arabia, Turkey, Our company always provides good quality and reasonable price for our customers. In our efforts, we already have many shops in Guangzhou and our products have won praise from customers worldwide. Our mission has always been simple: To delight our customers with best quality hair products and deliver on time. Welcome new and old customers to contact us for the future long term business relationships.
  • This enterprise in the industry is strong and competitive, advancing with the times and develop sustainable, we are very pleased to have a opportunity to cooperate!
    5 Stars By Doris from South Korea - 2017.10.13 10:47
    In China, we have many partners, this company is the most satisfying to us, reliable quality and good credit, it is worth appreciation.
    5 Stars By Astrid from Greek - 2017.08.21 14:13
    Write your message here and send it to us