Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya

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"Quality 1st, Honesty as base, Sincere company and mutual profit" is our idea, in an effort to create consistently and pursue the excellence for Primer Purification Methods, Dna Purification Column, Synthetic Empty Column, As a leading manufacture and exporter, we enjoy a good reputation in the international markets, especially in America and Europe, because of our top quality and reasonable prices.
Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


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We know that we only thrive if we can guarantee our combined price competiveness and quality advantageous at the same time for Good Quality Purification Instrument - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Italy, Malta, Mauritius, Our qualified engineering team will usually be prepared to serve you for consultation and feedback. We've been able to also deliver you with absolutely free samples to meet your needs. Best efforts might be made to offer you the ideal service and items. For anyone who is interested in our company and products, be sure to make contact with us by sending us emails or contact us right away. In order to know our solutions and organization. ar more, you can come to our factory to determine it. We are about to usually welcome guests from around the globe to our corporation. o create small business relations with us. Please genuinely feel no cost to speak to us for enterprise. nd we believe we have been gonna share the most effective trading practical experience with all our merchants.
  • We are really happy to find such a manufacturer that ensuring product quality at the same time the price is very cheap.
    5 Stars By Victoria from Montpellier - 2017.06.16 18:23
    This company conforms to the market requirement and joins in the market competition by its high quality product, this is an enterprise that have Chinese spirit.
    5 Stars By Grace from Chile - 2018.02.08 16:45
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