China wholesale Nucleic Acid Purification - Purification Equipment for Oligo purification – Honya

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China wholesale Nucleic Acid Purification - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


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Our advantages are lessen charges,dynamic income team,specialized QC,sturdy factories,premium quality services for China wholesale Nucleic Acid Purification - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Mongolia, Curacao, Hungary, Nowadays our products sell all over the domestic and abroad thanks for the regular and new customers support. We provide high quality product and competitive price, welcome the regular and new customers cooperate with us!
  • The company leader recept us warmly, through a meticulous and thorough discussion, we signed a purchase order. Hope to cooperate smoothly
    5 Stars By Vanessa from Cairo - 2018.06.18 17:25
    The quality of the products is very good, especially in the details, can be seen that the company work actively to satisfy customer's interest, a nice supplier.
    5 Stars By Jocelyn from Zurich - 2017.06.29 18:55
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