2022 wholesale price C 18 Purification - Purification Equipment for Oligo purification – Honya

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We strive for excellence, services the customers", hopes to be the top cooperation team and dominator business for personnel, suppliers and prospects, realizes benefit share and continual promotion for Nucleic Acid Synthesis Reagents, Small Nuclear Rna, Molecular Biology Reagent, At our firm with quality first as our motto, we manufacture products that are entirely made in Japan, from materials procurement to processing. This enables them to be used with confident peace of mind.
2022 wholesale price C 18 Purification - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


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We pursue the administration tenet of "Quality is superior, Services is supreme, Standing is first", and will sincerely create and share success with all customers for 2022 wholesale price C 18 Purification - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Ecuador, Nepal, Luxembourg, Due to good quality and reasonable prices, our products have been exported to more than 10 countries and regions. We are looking forward to cooperating with all customers from at home and abroad. Moreover, customer satisfaction is our eternal pursuit.
  • The company keeps to the operation concept "scientific management, high quality and efficiency primacy, customer supreme", we have always maintained business cooperation. Work with you,we feel easy!
    5 Stars By Beryl from Cambodia - 2017.03.28 16:34
    High Quality, High Efficiency, Creative and Integrity, worth having long-term cooperation! Looking forward to the future cooperation!
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