2022 wholesale price C 18 Purification - Purification Equipment for Oligo purification – Honya

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Innovation, good quality and reliability are the core values of our enterprise. These principles today extra than ever form the basis of our success as an internationally active mid-size organization for Page Purified Oligos, Nucleic Acid Synthesizer, 48 Channel Synthesizer, Seeing believes! We sincerely welcome the new clients abroad to build organization associations and also hope to consolidate the associations while using the long-established prospects.
2022 wholesale price C 18 Purification - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards 1, 3, 5, 8.
Filtration blow filtration, suction filtration
Number of injection ports  5, 6, 7, 8, 9, 10.
Compatible plate types  C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module single axis or dual axis
Voltage 220V
Warranty 1 year
Custom Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.


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We emphasize enhancement and introduce new solutions into the market just about every year for 2022 wholesale price C 18 Purification - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Jakarta, Poland, Bolivia, Wide selection and fast delivery for you! Our philosophy: Good quality, great service, keep improving. We are looking forward that more and more oversea friends join in our family for further development near the future!
  • A nice supplier in this industry, after a detail and careful discussion, we reached a consensus agreement. Hope that we cooperate smoothly.
    5 Stars By Martina from United Arab emirates - 2017.06.29 18:55
    Although we are a small company, we are also respected. Reliable quality, sincere service and good credit, we are honored to be able to work with you!
    5 Stars By Odelia from Macedonia - 2018.12.11 11:26
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