2022 High quality Primer Purification Instrument - Purification Equipment for Oligo purification – Honya

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Our business puts emphasis on the administration, the introduction of talented personnel, as well as the construction of team building, trying hard to further improve the standard and liability consciousness of staff members customers. Our enterprise successfully attained IS9001 Certification and European CE Certification of Dna Synthesis Without Base Protection, Nucleic Acid Protein Synthesis, Primer Making Machine, Our company quickly grew in size and reputation because of its absolute dedication to high quality manufacturing, high value of products and excellent customer service.

2022 High quality Primer Purification Instrument - Purification Equipment for Oligo purification – Honya Detail:

Specification

Compatible boards	1, 3, 5, 8.
Filtration	blow filtration, suction filtration
Number of injection ports	5, 6, 7, 8, 9, 10.
Compatible plate types	C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate
Module	single axis or dual axis
Voltage	220V
Warranty	1 year
Custom	Accepted

Purification Equipment for Oligo purification news3

Different ways of Purification

1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.

2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.

This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.

3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.

This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.

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To create much more price for clients is our company philosophy; purchaser growing is our working chase for 2022 High quality Primer Purification Instrument - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Latvia, Morocco, South Korea, By continuous innovation, we will provide you with more valuable products and services, and also make a contribution for the development of the automobile industry at home and abroad. Both domestic and foreign merchants are strongly welcomed to join us to grow together.

C 18 Purification